Principal Investigator Marion Jasnin
The Cryoskeleton Lab

Research

Our group uses in situ cryo-electron tomography methodologies, including correlative cryo-focused ion beam milling sample preparation and image processing to reveal actin architectures directly inside the native cellular environment. We explore the diversity of actin structures involved in invasion, including podosomes (Jasnin et al. Nat Commun 2022), invadopodia, and other actin-propelled membrane protrusions, as well as in cell contractility (Burbaum, Schneider et al. Nat Commun 2021). We develop computational tools for in-cell structural analysis of actin organization. For all these exciting projects, we collaborate with international teams of biologists, biophysicists and biochemists to combine structural, molecular, cellular and theoretical approaches. 

Our aims

To understand how actin, a small globular protein, and its associated cytoskeletal proteins can build a spectacular variety of complex filamentous structures inside cells, in order to advance our understanding of cell biology and disease mechanisms.

Current grants

Chan Zuckerberg initiative - Cryo-CLEM labels grant
DFG – ANR-DFG French-German call for joint projects in natural, life and engineering sciences
MIC Cancer Inserm – ITMO Aviesan call

Collaborative Projects

  • Macrophage podosomes – In collaboration with Renaud Poincloux / IPBS and Mira Krendel / SUNY Upstate Medical University
  • Multiscale modeling of invadosome force sensing – In collaboration with Matthieu Chavent and Renaud Poincloux / IPBS, Serge Dmitrieff and Antoine Jégou / Institut Jacques Monod, and Jean-Philip Piquemal / Sorbonne University
  • Tumor cell invadopodia – In collaboration with Philippe Chavrier / Institut Curie
  • Cell-cell fusion – In collaboration with Elizabeth Chen / UT Southwestern Medical Center
  • Deep learning-based segmentation tools – In collaboration with Tingying Peng / Helmholtz AI
  • Synthetic cell division – In collaboration with Petra Schwille / Max Planck Institute of Biochemistry

Dr. Marion Jasnin

Principal investigator, Cryoskeleton Lab

Marion received her PhD in Biophysics from Joseph Fourier University in 2007 under Professor Giuseppe Zaccai, where she exploited neutron scattering to explore water dynamics and macromolecular motions directly inside living cells. Her early postdoctoral work in the Department of Professor Wolfgang Baumeister at the Max Planck Institute of Biochemistry was supported by two postdoctoral fellowships (EMBO and Marie Curie fellowships, 2011-2013), and the award of an HFSP Program Grant (2016-2021) gave her the opportunity to start her own subgroup. With Renaud Poincloux / IPBS, she then obtained an ANR-DFG grant (cryoAdhesome, 2020-2024) to establish podosomes as a model system for structure-function analysis of the actin cytoskeleton in a cellular system that has direct implications for in vivo function, such as during bone turnover, tissue and cancer invasion. She also recently built a consortium with teams from the Institut Jacques-Monod, LPTMS and Sorbonne University to model podosome mechanics from the molecular scale up to the mesoscale, and were awarded an MIC grant (2020-2023). In 2022, she joined the Helmholtz Pioneer Campus as a Principal Investigator.

Factsheet

Dr. Marion Jasnin

Google Scholar

2022-present
Principal Investigator – Helmholtz Pioneer Campus, Helmholtz Munich

2017-2022
Project Group Leader – Max Planck Institute of Biochemistry

2010-2016
Postdoc (Wolfgang Baumeister) – Max Planck Institute of Biochemistry

2008-2009
Postdoc (Giuseppe Zaccai) – Institut Laue-Langevin (ILL)

2004-2007
Graduate Student (Giuseppe Zaccai and Moevea Tehei) – Institut de Biologie Structurale (IBS)

2004
Master Student (Daniel Riveline) – Laboratoire Interdisciplinaire de Physique (LIPhy)

2003
Student Intern (Nils O. Petersen) – Western University, Department of Chemistry

 

2007
PhD in Biophysics – Joseph Fourier University, Department of Physics

2004
MSc in Materials Physics – Joseph Fourier University, Department of Physics

2004
Diplôme National d’Ingénieur in Physics – Grenoble INP - Phelma

 

2016-2021
Participation in an HFSP Program Grant (Grant number RGP0035/2016)

2012-2013
Marie Curie Intra-European Fellowship (Grant number 273192)

2011
EMBO Long-Term Fellowship (Grant number 1099-2010)

2008-2009
ILL Postdoctoral Fellowship

2004-2007
Graduate Research Fellowship from the French Ministry of Education
Grenoble INP Graduate Lecturer Fellowship

2003-2004
Joseph Fourier University Masters Scholarship

 

2024
CZI Cryo-CLEM labels grant - CryoEMcapsulins

2020-2024
ANR-DFG NLE 2020 – Individual Research Grant (CryoAdhesome, Grant number JA-3038/2-1)

2020-2023
ITMO Cancer Aviesan call 2020 – Participation in an MIC Grant (Multiscale Modelling of Invadosome Force Sensing)

 

Software Scientist

Brice Beinsteiner

PhD in Structural Biology, Strasbourg University
Focus: Image processing, cryo-FIB/cryo-ET
PhD Thesis: "Origin and evolution of nuclear receptors and structural study of the first steroid, ERR"

PhD Students

Christina Correll

E-Mail

BSc in Biology, LMU Munich
Focus: 3D-correlative FIB milling
Bachelor Thesis: "The role of high-resolution electron microscopy in times of Corona and Ebola"

Thomas Drexler

E-Mail

MSc in Biophysics, Technical University Munich
Focus: Image analysis, cryo-FIB/cryo-ET
Master Thesis: "Cyclic linear transport and directed motion in DNA Origami Devices"

Amelie Niedermeier

MSc in Biochemistry and Molecular Biology, University of Bayreuth 
Focus: cryo-FIB/cryo-ET
Master Thesis: "Functional and structural characterization of the modulation of human sirtuins 3 and 5 by small molecules"

Jonathan Schneider

E-Mail

MSc in Biochemistry, LMU Munich
Focus: Subtomogram averaging, polarity analysis
Master Thesis: "The molecular architecture of human macrophage podosomes studied by cryo-electron tomography"

Lab Manager

Martin Warmer

E-Mail

BTA Biological Technician
Focus Lab organization, Light- and Electron Imaging

Selected Publications


In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography

Weimin Li,  Angdi Li, Bing Yu, Xiaoxiao Zhang, Xiaoyan Liu, Kate White, Raymond Stevens, Wolfgang Baumeister, Andrej Sali, Marion Jasnin, Liping Sun, Nat Commun. 2024 15:1311

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Molecular-scale visualization of sarcomere contraction within native cardiomyocytes

Laura Burbaum, Jonathan Schneider, Sarah Scholze, Ralph T Böttcher, Wolfgang Baumeister, Petra Schwille,  Jürgen M Plitzko, Marion Jasnin, Nat Commun. 2021 12:4086

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Elasticity of podosome actin networks produces nanonewton protrusive forces

Marion Jasnin, Jordan Hervy, Stéphanie Balor, Anaïs Bouissou, Amsha Proag, Raphaël Voituriez, Jonathan Schneider, Thomas Mangeat, Isabelle Maridonneau-Parini, Wolfgang Baumeister, Serge Dmitrieff, Renaud Poincloux. Nat Commun. 2022 13:3842

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Capturing actin assemblies in cells using in situ cryo-electron tomography

Jonathan Schneider, Marion Jasnin, Eur J Cell Biol. 2022 101:151224

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Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective

Saikat Chakraborty, Marion Jasnin, Wolfgang Baumeister, Protein Sci. 2020 29:1302-20

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The architecture of traveling actin waves revealed by cryo-electron tomography

Marion Jasnin, Florian Beck, Mary Ecke, Yoshiyuki Fukuda, Antonio Martinez-Sanchez, Wolfgang Baumeister, Günther Gerisch, Structure. 2019 27:1211-23.e5

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Actin organization in cells responding to a perforated surface, revealed by live imaging and cryo-electron tomography

Marion Jasnin, Mary Ecke, Wolfgang Baumeister, Günther Gerisch, Structure. 2016 24:1031-43

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Reversible membrane pearling in live cells upon destruction of the actin cortex

Doris Heinrich, Mary Ecke, Marion Jasnin, Ulrike Engel, Günther Gerisch, Biophys J. 2014 106:1079-91

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Three-dimensional architecture of actin filaments in Listeria monocytogenes comet tails

Marion Jasnin, Shoh Asano, Edith Gouin, Reiner Hegerl, Jürgen M Plitzko, Elizabeth Villa, Pascale Cossart, Wolfgang Baumeister, Proc Natl Acad Sci U S A. 2013 10:20521-6

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Contact us

HPC contact Jasnin

Contact

 

Helmholtz Pioneer Campus
Helmholtz Zentrum München
Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)

Ingolstädter Landstr. 1
85764 Neuherberg
Germany